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kapa hypercapture reagent kit  (Roche)


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    Structured Review

    Roche kapa hypercapture reagent kit
    Kapa Hypercapture Reagent Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kapa+hypercapture+reagent+kit/KAPA+Quantification+Kit/pmc12813098-89-28-32
    Average 99 stars, based on 5 article reviews
    kapa hypercapture reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Control:

    Article Title: Beyond tumor mutation burden: tumor neoantigen burden as a superior prognostic biomarker in resected gastric cancer
    Article Snippet: .. After quality control and quantification by 4200 TapeStation system (Agilent Technologies) and Flex fluorometer (Invitrogen), a total of 500 ng library distributed between 280–350 bp was captured by KAPA HyperCapture Reagent kit (Roche) and sequenced on Illumina NovaSeq6000 platform (Illumina) at a depth greater than 150X. ..

    Hybridization:

    Article Title: Nanoscale dynamics of enhancer–promoter interactions during exit from pluripotency
    Article Snippet: .. For the enrichment of fragments containing the viewpoints of interest, two subsequent hybridization reactions were performed using the KAPA HyperCapture Reagent kit (Roche, 9075828001) with biotinylated single-stranded DNA (ssDNA) probes complementary to the regions of interest. .. The ssDNA probes used for the Capture reactions were 100 nt long , designed using the oligo 0.2.0 python package ( oligo.readthedocs.io/en/latest/ ) and ordered from Integrated DNA Technologies as two xGen TM MRD Hybridization panels.

    Article Title: Nanoscale dynamics of enhancer–promoter interactions during exit from pluripotency
    Article Snippet: For the first hybridization reaction, 2 μg of each uniquely indexed library were combined in 1:1 mass ratio with 5 μl per library mouse Cot-1 DNA (Thermo Fisher Scientific, 18440016) and desiccated at 45°C in a vacuum centrifuge. .. The DNA pellet was then resuspended in 6.7 μl per library Universal Enhancing Oligonucleotides, 14 μl per library of Hybridization buffer, 6 μl per library Hybridization Component H and 4.5 μl per library diluted biotinylated oligonucleotides (2.9 nM per probe) and incubated at 95°C for 5 min and at 47°C for 72 h. Pulldown of the viewpoint-containing fragments was performed with 51 μl of M-270 streptavidin Dynabeads (Thermo Fisher Scientific, 65306) per library and using the KAPA HyperCapture Reagent kit (Roche, 9075828001). ..

    Article Title: Nanoscale dynamics of enhancer-promoter interactions during exit from pluripotency.
    Article Snippet: .. For the enrichment of fragments containing the viewpoints of interest, two subsequent hybridization reactions were performed using the KAPA HyperCapture Reagent kit (Roche, 9075828001) with biotinylated single-stranded DNA (ssDNA) probes complementary to the regions of interest. .. The ssDNA probes used for the Capture reactions were 100 nt long ( Supplementary Table S7 ), designed using the oligo 0.2.0 python package ( oligo.readthedocs.io/ en/ latest/ ) and ordered from Integrated DNA Technologies as two xGen TM MRD Hybridization panels.

    Article Title: Nanoscale dynamics of enhancer-promoter interactions during exit from pluripotency.
    Article Snippet: For the first hybridization reaction, 2 μg of each uniquely indexed library were combined in 1:1 mass ratio with 5 μl per library mouse Cot-1 DNA (Thermo Fisher Scientific, 18440016) and desiccated at 45 ◦C in a vacuum centrifuge. .. The DNA pellet was then resuspended in 6.7 μl per library Universal Enhancing Oligonucleotides, 14 μl per library of Hybridization buffer, 6 μl per library Hybridization Component H and 4.5 μl per library diluted biotinylated oligonucleotides (2.9 nM per probe) and incubated at 95 ◦C for 5 min and at 47 ◦C for 72 h. Pulldown of the viewpoint-containing fragments was performed with 51 μl of M-270 streptavidin Dynabeads (Thermo Fisher Scientific, 65306) per library and using the KAPA HyperCapture Reagent kit (Roche, 9075828001). ..

    Incubation:

    Article Title: Nanoscale dynamics of enhancer–promoter interactions during exit from pluripotency
    Article Snippet: For the first hybridization reaction, 2 μg of each uniquely indexed library were combined in 1:1 mass ratio with 5 μl per library mouse Cot-1 DNA (Thermo Fisher Scientific, 18440016) and desiccated at 45°C in a vacuum centrifuge. .. The DNA pellet was then resuspended in 6.7 μl per library Universal Enhancing Oligonucleotides, 14 μl per library of Hybridization buffer, 6 μl per library Hybridization Component H and 4.5 μl per library diluted biotinylated oligonucleotides (2.9 nM per probe) and incubated at 95°C for 5 min and at 47°C for 72 h. Pulldown of the viewpoint-containing fragments was performed with 51 μl of M-270 streptavidin Dynabeads (Thermo Fisher Scientific, 65306) per library and using the KAPA HyperCapture Reagent kit (Roche, 9075828001). ..

    Article Title: Nanoscale dynamics of enhancer-promoter interactions during exit from pluripotency.
    Article Snippet: For the first hybridization reaction, 2 μg of each uniquely indexed library were combined in 1:1 mass ratio with 5 μl per library mouse Cot-1 DNA (Thermo Fisher Scientific, 18440016) and desiccated at 45 ◦C in a vacuum centrifuge. .. The DNA pellet was then resuspended in 6.7 μl per library Universal Enhancing Oligonucleotides, 14 μl per library of Hybridization buffer, 6 μl per library Hybridization Component H and 4.5 μl per library diluted biotinylated oligonucleotides (2.9 nM per probe) and incubated at 95 ◦C for 5 min and at 47 ◦C for 72 h. Pulldown of the viewpoint-containing fragments was performed with 51 μl of M-270 streptavidin Dynabeads (Thermo Fisher Scientific, 65306) per library and using the KAPA HyperCapture Reagent kit (Roche, 9075828001). ..



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